Strawberry DNA ExtractionCourtesy of Ms. Templin
- What is DNA?
- Where is DNA found?
- Does DNA have the same basic structure from all oranisms?
To extract DNA from the fruit of a strawberry plant.
- Do not eat or drink in the laboratory.
- Use safety goggles
- 1 Ziploc bag
- 1 strawberry
- graduated cylinder
- 100 mL beaker
- wooden coffee stirrer
- DNA extraction buffer
- 95% Ethanol
- Place one strawberry in a Ziploc bag.
- Smash strawberry with hands for 2 minutes. Make sure the bag does not break.
- Add 10 mL of the extraction buffer to the bag.
- Mush again for 1 minute.
- Fliter through the cheese cloth in a funnel into the beaker.
- Pour the filtrate into the test tube so that it is 1/8 full
- Slowly pour the ice-cold ethanol into the test tube until it is half full.
- At the interface, you will see the DNA precipitate out of solution and float to the top.
- You may spool the DNA on the end of a glass rod.
- Where can DNA be found in the cell?
- Discuss the action of the soap (detergent) on the cell. What is the purpose of the soap in this activity?
- What was the purpose of the Sodium Chloride? Include a discussion of polarity and charged particles.
- Why was the cold ethanol added to the soap and salt mixture?
- Describe the appearance of your final product?
- Draw a diagram of DNA containing 5 sets of nucleotide bases labeling the hydrogen bonds between the bases.